| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kunkel, William H (12)
|
MDLBA |
42.43 |
39.63 |
-2.80 |
| 2 |
Ddungu, Hashir I (12)
|
MDEST |
44.47 |
42.28 |
-2.19 |
| 3 |
Mislow, Max K (12)
|
MDEST |
50.24 |
42.96 |
-7.28 |
| 4 |
Kong, Matthew (12)
|
MDEST |
50.06 |
44.20 |
-5.86 |
| 5 |
Li, Ryan (11)
|
MDEST |
46.73 |
44.35 |
-2.38 |
| 6 |
Siglar, Bradey A (12)
|
MDHSST |
1:02.25 |
45.31 |
-16.94 |
| 7 |
White, Noah M (12)
|
MDHSST |
46.61 |
47.39 |
0.78 |
| 8 |
Speace, Hayden H (12)
|
MDEST |
53.05 |
47.40 |
-5.65 |
| 9 |
Skidmore, Holden D (11)
|
MDEST |
48.62 |
47.49 |
-1.13 |
| 10 |
Navarra, Niall J (11)
|
MDMAS |
53.54 |
47.57 |
-5.97 |
| 11 |
Celtnieks, Eli H (11)
|
MDLBA |
56.94 |
48.00 |
-8.94 |
| 12 |
Stanco, David M (12)
|
MDMSC |
52.63 |
48.49 |
-4.14 |
| 13 |
Risley, Elijah D (11)
|
MDHSST |
48.86 |
48.99 |
0.13 |
| 14 |
Brazhnikov, Nicholas R (11)
|
MDMSC |
49.17 |
49.18 |
0.01 |
| 14 |
Missry, Leon A (12)
|
MDMAS |
54.74 |
49.18 |
-5.56 |
| 16 |
Liang, Kai H (11)
|
MDEST |
58.58 |
49.44 |
-9.14 |
| 17 |
Farls, Benjamin A (11)
|
MDLBA |
48.19 |
50.18 |
1.99 |
| 18 |
Kim, Jonathan J (12)
|
MDEST |
52.60 |
51.03 |
-1.57 |
| 19 |
Webbert, Jeremiah T (11)
|
MDLBA |
52.24 |
51.61 |
-0.63 |
| 20 |
Kay, Tyler O (11)
|
MDEST |
52.14 |
52.13 |
-0.01 |
| 21 |
Woldeselassie, Nathan M (12)
|
MDEST |
53.46 |
52.30 |
-1.16 |
| 22 |
Vong, Leo S (11)
|
MDMAS |
1:04.73 |
52.57 |
-12.16 |
| 23 |
Li, Nathan C (11)
|
MDEST |
53.79 |
55.31 |
1.52 |
| 24 |
Di Gregorio, Caleb E (11)
|
MDMAS |
1:05.38 |
55.96 |
-9.42 |
| 25 |
Ko, Martin (11)
|
MDEST |
58.12 |
56.04 |
-2.08 |
| 26 |
Znamirowski, Jason W (12)
|
MDMAS |
|
56.13 |
- |
| 27 |
Lepley, Carter W (11)
|
MDLBA |
1:18.41 |
56.48 |
-21.93 |
| 28 |
Kennedy, Tripp L (11)
|
MDSPY |
1:03.47 |
56.65 |
-6.82 |
| 29 |
Lynch, Adam N (11)
|
MDLBA |
59.11 |
59.02 |
-0.09 |
| 30 |
Romerio, Antonio B (11)
|
MDMSC |
1:11.46 |
1:02.19 |
-9.27 |
|
Pereira, Alex (12)
|
MDMAS |
|
DQ |
- |
|
Monahan, Sean P (11)
|
MDSPRC |
|
DQ |
- |