| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Leibson, Charles D (19)
|
OHPCY |
2:39.95 |
2:42.31 |
2.36 |
| 2 |
Haaksma, Jacob A (15)
|
MIRAYS |
2:50.18 |
2:43.14 |
-7.04 |
| 3 |
Sherman, Adam J (16)
|
OHPCY |
2:25.62 |
2:43.36 |
17.74 |
| 4 |
Fetterhoff, Elijah J (14)
|
MIRAYS |
2:59.28 |
2:44.70 |
-14.58 |
| 5 |
Fulton, Ezekiel J (19)
|
OHMCY |
2:38.15 |
2:51.31 |
13.16 |
| 6 |
Roberts, Cameron L (14)
|
OHKEY |
2:48.55 |
2:51.43 |
2.88 |
| 7 |
Nyberg, Quinn R (17)
|
OHPCY |
2:56.18 |
2:51.72 |
-4.46 |
| 8 |
Feeney, Jameson D (16)
|
LARRAC |
2:52.73 |
2:54.28 |
1.55 |
| 9 |
Lucas, Kyle (16)
|
ZZAMFY |
2:48.87 |
2:58.39 |
9.52 |
| 10 |
Day, Dylan S (14)
|
OHSPY |
3:01.75 |
2:59.28 |
-2.47 |
| 11 |
Koetter, Alexander E (15)
|
OHBASH |
2:57.12 |
2:59.67 |
2.55 |
| 12 |
Kaising, Logan A (15)
|
OHBASH |
|
3:01.34 |
- |
| 13 |
Platt, Ryoma T (13)
|
OHABLY |
3:04.80 |
3:01.46 |
-3.34 |
| 14 |
Luebke, Griffin (12)
|
ZZAMFY |
3:04.10 |
3:04.58 |
0.48 |
| 15 |
Weisfelder, Jonathan K (16)
|
OHBASH |
2:53.11 |
3:05.34 |
12.23 |
| 16 |
Cangiano, Peter L (14)
|
OHBASH |
|
3:07.79 |
- |
| 17 |
Zhao, Jason M (11)
|
OHBASH |
3:37.84 |
3:09.28 |
-28.56 |
| 18 |
Valentine, Joshua M (15)
|
OHPCY |
|
3:16.81 |
- |
| 19 |
Kenerly, Christopher J (16)
|
OHPCY |
3:23.91 |
3:16.97 |
-6.94 |
| 20 |
Bogen, Joshua D (13)
|
OHPCY |
3:26.84 |
3:21.09 |
-5.75 |
| 21 |
Smal, Fredrik F (14)
|
OHRCDB |
3:23.01 |
3:24.49 |
1.48 |
| 22 |
Lewis, Robert K (10)
|
OHBASH |
|
3:31.86 |
- |
| 23 |
Bachman, Griffin J (18)
|
OHPCY |
|
3:32.75 |
- |
| 24 |
Grider, Ryan J (14)
|
OHPCY |
3:30.64 |
3:33.70 |
3.06 |
|
Clark, Nathaniel G (14)
|
OHPCY |
|
NS |
- |
|
Heffron, Samuel S (17)
|
OHPCY |
|
NS |
- |
|
Kelley, Griffin T (16)
|
OHPCY |
|
NS |
- |
|
Pendery, Greyson T (19)
|
OHPCY |
2:56.97 |
NS |
- |
|
Ferrara, Owen R (14)
|
OHABLY |
|
NS |
- |
|
Grant, Nate J (16)
|
OHBASH |
|
NS |
- |