| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cech, Hannah N (16)
|
OHLAKY |
2:38.79 |
2:30.76 |
-8.03 |
| 2 |
Holt, Sarah E (15)
|
LARRAC |
2:22.85 |
2:31.39 |
8.54 |
| 3 |
Johnson, Alexandra F (13)
|
OHABLY |
2:48.25 |
2:43.22 |
-5.03 |
| 4 |
Smith, Sarah Grace G (17)
|
OHBASH |
|
2:43.70 |
- |
| 5 |
Valentine, Dominique M (17)
|
OHPCY |
2:33.19 |
2:47.42 |
14.23 |
| 6 |
Callard, Dorothy J (16)
|
OHPCY |
|
2:50.85 |
- |
| 7 |
Johnson, Lucia M (13)
|
OHABLY |
2:47.49 |
2:55.67 |
8.18 |
| 8 |
Smith, Annalise M (13)
|
OHBASH |
|
3:03.59 |
- |
| 9 |
Glaser, Gracie J (16)
|
OHSPY |
3:20.86 |
3:04.91 |
-15.95 |
| 10 |
Gomez, Isabelle (13)
|
OHLAKY |
|
3:06.11 |
- |
| 11 |
Krzywanski, Melissa G (15)
|
LARRAC |
3:03.01 |
3:08.36 |
5.35 |
| 12 |
Groseclose, Megan P (13)
|
OHLAKY |
3:20.97 |
3:16.98 |
-3.99 |
| 13 |
McCann, Lahna J (14)
|
OHPCY |
|
3:25.67 |
- |
|
Constantinides, Chloe P (15)
|
OHPCY |
2:53.59 |
DQ |
- |
|
Phifer, Ciara P (13)
|
OHPCY |
|
NS |
- |
|
Reed, Emma C (17)
|
OHPCY |
|
NS |
- |
|
Robles, Eva B (12)
|
OHPCY |
|
NS |
- |
|
Elliott, Mikaela A (15)
|
OHPCY |
2:50.21 |
NS |
- |
|
Anderson, Julia C (15)
|
OHMCY |
|
NS |
- |