| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reynolds, Ashley L (10)
|
MDCAC |
53.43 |
45.99 |
-7.44 |
| 2 |
Jones, Emily L (10)
|
MDHOCO |
|
48.21 |
- |
| 3 |
Eversole, Jayden M (10)
|
MDCAC |
|
49.29 |
- |
| 4 |
Garner, Luna E (10)
|
MDCAC |
|
52.35 |
- |
| 5 |
Lisa, Anna K (9)
|
MDROCK |
|
56.24 |
- |
| 6 |
de la Cerda, Anastasia M (9)
|
MDCAC |
|
57.95 |
- |
| 7 |
Mulugeta, Hanna S (10)
|
MDHOCO |
|
58.04 |
- |
| 8 |
Longmore, Gabrielle L (10)
|
MDGMAC |
57.70 |
58.28 |
0.58 |
| 9 |
Karunwi, Aderinsola O (10)
|
MDHOCO |
|
1:05.95 |
- |
| 10 |
Arias, Mia F (10)
|
MDCAC |
|
1:12.05 |
- |
|
Denison, Abigail F (9)
|
MDCAC |
|
DQ |
- |
|
Shoots, Maia G (10)
|
MDHOCO |
|
DQ |
- |
|
Lederman, Elena R (10)
|
MDHOCO |
55.62 |
NS |
- |