| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grafmiller, Kylee R (14)
|
GUBTA |
2:34.15 |
2:35.94 |
1.79 |
| 2 |
Kitchel, Kassidy G (13)
|
GUDADS |
2:33.53 |
2:39.21 |
5.68 |
| 3 |
Ortiz, Allison C (13)
|
GUDADS |
2:42.77 |
2:43.72 |
0.95 |
| 4 |
Vidinha, Alexandra J (14)
|
GUSHRK |
3:08.05 |
2:44.08 |
-23.97 |
| 5 |
Burke, Klara M (13)
|
GUPEAK |
2:44.20 |
2:47.68 |
3.48 |
| 6 |
Birkelbach, Stephanie Y (13)
|
GUDADS |
2:21.86 |
2:47.86 |
26.00 |
| 7 |
Cole, Daria R (13)
|
GUSSS |
2:42.26 |
2:48.18 |
5.92 |
| 8 |
Becker, Peyton R (14)
|
GUSHRK |
3:07.43 |
2:49.38 |
-18.05 |
| 9 |
Valenzuela, Sophia E (13)
|
GUBTA |
2:25.94 |
2:49.49 |
23.55 |
| 10 |
Givens, Leah M (14)
|
GUSHRK |
3:04.60 |
2:50.04 |
-14.56 |
| 11 |
Wong, Lindsay H (13)
|
GUDADS |
2:37.91 |
2:53.68 |
15.77 |
| 12 |
Fagen, Meredith G (13)
|
GUBTA |
2:44.39 |
2:54.27 |
9.88 |
| 13 |
Bentley, Elaine E (14)
|
GUSHRK |
2:32.57 |
2:54.63 |
22.06 |
| 14 |
Huang, Anna J (13)
|
GUSSS |
2:52.60 |
2:55.48 |
2.88 |
| 15 |
Linden, Alexandra E (14)
|
GUSHRK |
2:54.64 |
2:56.65 |
2.01 |
| 16 |
Vestal, Emma L (13)
|
GUSHRK |
2:54.30 |
2:58.30 |
4.00 |
| 17 |
Nuval, Veronica M (14)
|
GUPEAK |
2:53.90 |
2:59.44 |
5.54 |
| 18 |
Walsh, Marie C (14)
|
GUDADS |
3:15.73 |
3:04.46 |
-11.27 |
| 19 |
Gutierrez, Tiffany A (14)
|
GUPEAK |
2:49.93 |
3:05.31 |
15.38 |
| 20 |
Rogers, Kaia G (13)
|
GUPEAK |
3:21.67 |
3:05.70 |
-15.97 |
| 21 |
Lau, Paysan H (14)
|
GUDADS |
|
3:10.49 |
- |
| 22 |
Dudley, Elizabeth C (13)
|
GUSSS |
3:20.47 |
3:15.82 |
-4.65 |
| 23 |
Burke, Ellie A (13)
|
GUSHRK |
2:48.10 |
3:16.21 |
28.11 |
| 24 |
Winson, Amelia A (14)
|
GUPEAK |
3:24.54 |
3:18.08 |
-6.46 |
| 25 |
Murillo, Melina (14)
|
GUPEAK |
3:27.77 |
3:18.98 |
-8.79 |
| 26 |
De Silva, Thinara (14)
|
GUPEAK |
|
4:09.06 |
- |