| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Flannery, Michael P (10)
|
NIGCOM |
41.03 |
38.89 |
-2.14 |
| 2 |
Zornow, Daniel T (10)
|
NIGCOM |
38.62 |
39.26 |
0.64 |
| 3 |
Kilchevskyy, Michael (10)
|
NISDSC |
38.91 |
39.79 |
0.88 |
| 4 |
Warren, Wesley C (9)
|
NIWEBS |
44.91 |
43.89 |
-1.02 |
| 5 |
Peters, Evan M (10)
|
NIGCOM |
45.80 |
44.17 |
-1.63 |
| 6 |
Bierley, Benjamin R (10)
|
NISDSC |
43.42 |
45.10 |
1.68 |
| 7 |
Natale, Anthony J (10)
|
NIHAST |
49.98 |
46.44 |
-3.54 |
| 8 |
Quigley, Kian C (10)
|
NIGRSC |
48.17 |
47.38 |
-0.79 |
| 9 |
Rank, Luciano M (10)
|
NINEWW |
47.15 |
48.09 |
0.94 |
| 10 |
Tang, Howard Z (9)
|
NIGCOM |
|
48.65 |
- |
| 11 |
Scamacca, Michael A (9)
|
NIHAST |
51.26 |
49.42 |
-1.84 |
| 12 |
Urminsky, Maxwell J (9)
|
NISDSC |
49.02 |
49.49 |
0.47 |
| 13 |
Kuchma, Michael N (10)
|
NIGRAT |
|
49.68 |
- |
| 14 |
Garnot, Griffin W (10)
|
NIWEBS |
46.75 |
50.34 |
3.59 |
| 15 |
Gensini, Alejandro (9)
|
NIVICT |
49.56 |
50.65 |
1.09 |
| 16 |
Napier, Alexander W (9)
|
NISDSC |
|
50.76 |
- |
| 17 |
Wood, Evan E (10)
|
NINEWW |
57.08 |
53.89 |
-3.19 |
| 18 |
Vallow, Brayden T (10)
|
NISDSC |
|
55.25 |
- |
| 19 |
McCarthy, John E (10)
|
NISDSC |
|
55.91 |
- |
| 20 |
Bondar, Vladimir D (9)
|
NIGCOM |
|
59.59 |
- |
| 21 |
DaGama, Michael F (10)
|
NIGCOM |
|
1:00.50 |
- |
| 22 |
Alello, Joseph P (10)
|
NISDSC |
57.96 |
1:00.73 |
2.77 |
| 23 |
Ellickson, Cameron B (10)
|
NISDSC |
|
1:05.19 |
- |
| 24 |
Potter, Logan C (9)
|
NIGRAT |
|
1:07.44 |
- |
| 25 |
Blatt, Quinlan J (9)
|
NISDSC |
|
1:09.00 |
- |
| 26 |
Goltsman, Adam J (10)
|
NISDSC |
1:04.86 |
1:11.27 |
6.41 |
| 27 |
McShanley, Brandon C (10)
|
NINEWW |
1:16.69 |
1:13.53 |
-3.16 |
|
Twohig, Colin M (9)
|
NIGRSC |
|
DQ |
- |
|
Hinz, Kaiden M (10)
|
NIWEBS |
|
DQ |
- |