| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kilchevskyy, Michael (11)
|
NIWEBS |
38.91 |
36.59 |
-2.32 |
| 2 |
Zornow, Daniel T (11)
|
NIQUIK |
38.62 |
37.97 |
-0.65 |
| 3 |
Lewis, Matthew K (11)
|
NIQUIK |
37.84 |
38.84 |
1.00 |
| 4 |
Anthony, Jaydon T (12)
|
NIPMSC |
41.37 |
42.14 |
0.77 |
| 5 |
Monroe, Lucas J (12)
|
NIPMSC |
43.62 |
44.93 |
1.31 |
| 6 |
Ellickson, Cameron B (11)
|
NISDSC |
47.22 |
47.79 |
0.57 |
| 7 |
Piedigrossi, Christopher J (12)
|
NIGCOM |
47.87 |
47.88 |
0.01 |
| 8 |
Passinault, Easton J (11)
|
NIGRSC |
51.40 |
48.78 |
-2.62 |
| 9 |
Fox, Johnathan M (11)
|
NISDSC |
49.14 |
50.57 |
1.43 |
| 10 |
Geoca, Andrew K (11)
|
NISDSC |
|
50.87 |
- |
| 11 |
Macomber, Tavis D (11)
|
NISDSC |
|
56.68 |
- |
|
Cordy, Brandon C (12)
|
NISDSC |
|
DQ |
- |
|
Pendri, Vinay R (12)
|
NIGCOM |
49.41 |
NS |
- |
|
Voytynskyy, Petro (12)
|
NIGRSC |
|
NS |
- |
|
Blancaflor, Aaron Joseph (11)
|
NIGCOM |
|
NS |
- |
|
Dwivedi, Arjun M (11)
|
NIGCOM |
49.73 |
NS |
- |
|
Hedden, Dominick J (11)
|
NIVICT |
56.41 |
NS |
- |
|
Cao, Son T (11)
|
NIGCOM |
|
NS |
- |