| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roncone, Aria R (7)
|
AMSFAC |
25.87 |
21.89 |
-3.98 |
| 2 |
Opitz, Lillian K (7)
|
AMPEAQ |
21.47 |
22.57 |
1.10 |
| 3 |
Turner, Alyssa J (7)
|
AMRACE |
24.59 |
25.44 |
0.85 |
| 4 |
Sinderson, Jessica L (7)
|
AMPEAQ |
27.29 |
25.85 |
-1.44 |
| 5 |
McKinney, Bailey R (7)
|
AMRACE |
28.51 |
27.45 |
-1.06 |
| 6 |
Bittner, Mia A (7)
|
AMRXA |
30.15 |
27.94 |
-2.21 |
| 7 |
Chandiran, Sai Anshika (7)
|
AMPEAQ |
29.29 |
28.70 |
-0.59 |
| 8 |
Robinson, Gabrielle D (7)
|
AMSFAC |
34.06 |
29.05 |
-5.01 |
| 9 |
Hartman, Caroline R (7)
|
AMPEAQ |
29.49 |
30.35 |
0.86 |
| 10 |
Mandel, Ryann C (7)
|
AMRXA |
45.99 |
33.73 |
-12.26 |
| 11 |
Pipes, Kayley G (7)
|
AMWAAC |
28.56 |
34.01 |
5.45 |
| 12 |
Ward, Ariana R (7)
|
AMWAAC |
33.48 |
36.98 |
3.50 |