| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schomaker, Charlotte J (7)
|
AMNHA |
43.19 |
43.28 |
0.09 |
| 2 |
Rees, Leia K (7)
|
AMNHA |
42.03 |
45.51 |
3.48 |
| 3 |
Lawrence, Rory C (7)
|
AMNHA |
48.56 |
46.22 |
-2.34 |
| 4 |
McGuire, Addison F (7)
|
AMPEAQ |
51.47 |
48.03 |
-3.44 |
| 5 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
52.98 |
48.86 |
-4.12 |
| 6 |
Capp, Molly L (7)
|
AMMOON |
53.68 |
49.64 |
-4.04 |
| 7 |
Obsincs, Gabrielle A (7)
|
AMNHA |
58.01 |
51.62 |
-6.39 |
| 8 |
Troiana, Riya I (7)
|
AMHD |
58.27 |
53.88 |
-4.39 |
| 9 |
Brunner, Tessa G (7)
|
AMPEAQ |
55.96 |
54.40 |
-1.56 |
| 10 |
Robinson, Gabrielle D (7)
|
AMSFAC |
1:05.17 |
56.89 |
-8.28 |
| 11 |
Chandiran, Sai Anshika (7)
|
AMPEAQ |
57.62 |
57.81 |
0.19 |
| 12 |
Hartman, Caroline R (7)
|
AMPEAQ |
1:06.63 |
1:05.31 |
-1.32 |
| 13 |
McKinney, Bailey R (7)
|
AMRACE |
1:09.79 |
1:05.32 |
-4.47 |
| 14 |
Page, Brynley J (7)
|
AMHD |
1:11.61 |
1:13.79 |
2.18 |
| 15 |
Connolly, Mae E (7)
|
AMPEAQ |
1:13.54 |
1:14.60 |
1.06 |
| 16 |
Berke, Mikayla R (7)
|
AMHD |
1:40.72 |
1:21.00 |
-19.72 |
|
Palkovitch, Scarlet M (7)
|
AMRACE |
1:23.14 |
NS |
- |
|
Ponder, Alacia M (7)
|
AMRACE |
1:19.17 |
NS |
- |
|
Ressler, Shyloh W (7)
|
AMPRA |
1:01.97 |
NS |
- |