| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rassiane, Aliya C (10)
|
AMMMSA |
2:27.46 |
2:26.33 |
-1.13 |
| 2 |
Daugherty, Amara Q (10)
|
AMANSC |
2:35.57 |
2:39.62 |
4.05 |
| 3 |
Jochims, Ava G (10)
|
AMRXA |
2:43.58 |
2:39.80 |
-3.78 |
| 4 |
Daugherty, Lillian N (10)
|
AMANSC |
2:32.17 |
2:39.91 |
7.74 |
| 5 |
Calhoun, Whitney J (9)
|
AMRXA |
2:52.90 |
2:48.78 |
-4.12 |
| 6 |
Munoz, Kimberly A (10)
|
AMSFAC |
3:18.88 |
2:59.82 |
-19.06 |
| 7 |
Tom, Anika M (10)
|
AMFAST |
3:02.64 |
3:00.42 |
-2.22 |
| 8 |
Bowers, Taylor J (9)
|
AMPTSC |
3:03.10 |
3:05.33 |
2.23 |
| 9 |
Kaminski, Elle K (10)
|
AMMMSA |
3:18.99 |
3:05.44 |
-13.55 |
| 10 |
Conner, Lyla B (10)
|
AMPTSC |
3:13.54 |
3:05.53 |
-8.01 |
| 11 |
Sullivan, Keelin A (10)
|
AMNAC |
3:15.53 |
3:07.30 |
-8.23 |
| 12 |
Cornell, Olivia R (10)
|
AMWAAC |
3:15.92 |
3:10.01 |
-5.91 |
| 13 |
Freshcorn, Rylee J (9)
|
AMRACE |
3:17.64 |
3:16.69 |
-0.95 |
| 14 |
Mariana, Elizabeth M (10)
|
AMKW |
3:16.64 |
3:19.70 |
3.06 |
| 15 |
McCarthy, Morgan M (10)
|
AMWAAC |
3:17.38 |
3:27.66 |
10.28 |
| 16 |
Baiano, Emalina L (9)
|
AMSFAC |
3:15.19 |
3:29.06 |
13.87 |
| 17 |
Barnes, Cloey U (9)
|
AMSFAC |
3:14.19 |
3:29.59 |
15.40 |
| 18 |
Shanley, Delaney A (9)
|
AMANSC |
3:40.70 |
3:34.61 |
-6.09 |
|
Burgess, Rebecca A (10)
|
AMFAST |
2:51.05 |
NS |
- |