| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaguzis, Peri S (14)
|
AMKW |
2:51.06 |
2:56.55 |
5.49 |
| 2 |
Pasquella, Ariana D (14)
|
AMKW |
3:19.12 |
3:08.70 |
-10.42 |
| 3 |
Sharp, Abigail L (14)
|
AMFAST |
3:16.87 |
3:17.42 |
0.55 |
| 4 |
Bliss, Evelyn (13)
|
AMUN-R |
2:21.89 |
3:19.47 |
57.58 |
| 5 |
Georgagis, Anastasia E (14)
|
AMBVSC |
3:10.25 |
3:22.32 |
12.07 |
| 6 |
Walko, Julia F (13)
|
AMNAC |
3:22.65 |
3:26.52 |
3.87 |
| 7 |
Pratt, Riley M (14)
|
AMKW |
3:14.04 |
3:27.00 |
12.96 |
| 8 |
Haas, Katherine R (13)
|
AMKW |
3:36.07 |
3:27.55 |
-8.52 |
| 9 |
Meyers, Mackenzie E (13)
|
AMEFSC |
3:14.41 |
3:28.05 |
13.64 |
| 10 |
LaRoche, Francesca L (13)
|
AMWHAT |
3:24.72 |
3:30.75 |
6.03 |
| 11 |
Morelock, Carolyn A (14)
|
AMSFAC |
3:44.89 |
3:31.63 |
-13.26 |
| 12 |
Gifford, Adelie T (14)
|
AMSVSC |
3:50.19 |
3:41.70 |
-8.49 |
| 13 |
Carey, Christina J (13)
|
AMSPAC |
3:25.00 |
3:45.71 |
20.71 |
| 14 |
Tucker, Angela M (13)
|
AMCSC |
4:09.14 |
3:46.26 |
-22.88 |
| 15 |
Bedell, Emma K (14)
|
AMSVSC |
3:40.02 |
3:51.11 |
11.09 |
| 16 |
Bixler, Willow T (14)
|
AMFAST |
4:25.99 |
3:53.79 |
-32.20 |
| 17 |
Sanders, Marayna G (13)
|
AMGCY |
3:45.22 |
3:55.19 |
9.97 |
| 18 |
Osullivan, Avery D (13)
|
AMTRIN |
4:20.33 |
3:57.94 |
-22.39 |