| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dantey, Ellie M (10)
|
AMKW |
42.96 |
41.27 |
-1.69 |
| 2 |
George, Olivia F (10)
|
AMNAC |
44.81 |
42.57 |
-2.24 |
| 3 |
Acerra, Shannon M (10)
|
AMHD |
45.39 |
43.76 |
-1.63 |
| 4 |
Brindza, Cameryn L (10)
|
AMKW |
47.16 |
44.13 |
-3.03 |
| 5 |
Shelkey-Bray, Maren J (10)
|
AMKW |
51.10 |
49.76 |
-1.34 |
| 6 |
Kozar, Elizabeth G (10)
|
AMSHAQ |
54.57 |
54.88 |
0.31 |
| 7 |
Newsome, Delaney A (10)
|
AMBWA |
58.16 |
55.17 |
-2.99 |
| 8 |
Sumrok, Cecilia A (10)
|
AMKW |
59.10 |
57.24 |
-1.86 |
| 9 |
Melocchi, Ava P (10)
|
AMSASC |
1:00.49 |
57.26 |
-3.23 |
| 10 |
Mack, Kayla M (10)
|
AMHD |
1:04.55 |
57.54 |
-7.01 |
| 11 |
LaBella, Kailyn M (10)
|
AMSASC |
58.87 |
58.03 |
-0.84 |
|
Ressler, Nicola L (10)
|
AMKW |
1:00.02 |
DQ |
- |
|
Foose, Paige E (10)
|
AMHD |
1:20.98 |
DQ |
- |
|
Federico, Teegan M (10)
|
AMBWA |
|
NS |
- |
|
Gregorowicz, Caroline O (10)
|
AMPRA |
55.27 |
NS |
- |