| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Opitz, Lauren E (12)
|
AMPEAQ |
3:11.13 |
3:01.99 |
-9.14 |
| 2 |
Roy, Sylvia M (12)
|
AMMLAC |
3:15.16 |
3:04.40 |
-10.76 |
| 3 |
Alder, Alexandra O (12)
|
AMNHA |
3:29.53 |
3:08.55 |
-20.98 |
| 4 |
Rothrock, Lena R (12)
|
AMSVSC |
3:11.16 |
3:10.00 |
-1.16 |
| 5 |
Alexander, Gabrielle C (12)
|
AMSVSC |
3:08.93 |
3:10.83 |
1.90 |
| 6 |
MacAdams, Ainsley L (11)
|
AMPEAQ |
3:18.12 |
3:11.37 |
-6.75 |
| 7 |
Kovach, Grace E (12)
|
AMKW |
|
3:15.06 |
- |
| 8 |
Reinhart, Margaret (Meg) T (12)
|
AMMLAC |
3:50.99 |
3:23.15 |
-27.84 |
| 9 |
Sutkowski, Emilia A (12)
|
AMKW |
3:40.02 |
3:23.68 |
-16.34 |
| 10 |
Allen, Julina M (12)
|
AMWAAC |
3:23.88 |
3:32.13 |
8.25 |
| 11 |
Dubreil, Eva S (12)
|
AMPEAQ |
3:37.98 |
3:32.26 |
-5.72 |
| 12 |
Vales, Sophia L (11)
|
AMANSC |
3:40.70 |
3:42.70 |
2.00 |
| 13 |
Berg, Camille R (11)
|
AMSFAC |
3:48.18 |
3:48.48 |
0.30 |
|
Ginsburg, Lauren E (11)
|
AMPEAQ |
4:10.44 |
DQ |
- |