| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crane, Abigail C (7)
|
AMANSC |
29.68 |
23.74 |
-5.94 |
| 2 |
Cessna, Adalynn M (7)
|
AMRGSY |
27.07 |
23.89 |
-3.18 |
| 3 |
Briggs, Tori R (7)
|
AM3RVS |
25.70 |
24.45 |
-1.25 |
| 4 |
DeMico, Nora M (7)
|
AMSTEE |
24.66 |
24.78 |
0.12 |
| 5 |
Cutone, Arianna R (7)
|
AMSTEE |
35.12 |
24.89 |
-10.23 |
| 6 |
Renner, Abigail M (7)
|
AMSTEE |
39.66 |
26.03 |
-13.63 |
| 7 |
Loutsenhizer, Amelia G (7)
|
AMPRA |
31.00 |
26.70 |
-4.30 |
| 8 |
Silvaggio, Josephine M (7)
|
AMPRA |
29.63 |
26.86 |
-2.77 |
| 9 |
Milliron, Riley M (7)
|
AM3RVS |
28.10 |
29.79 |
1.69 |
| 10 |
Davis, Daphne G (7)
|
AMPRA |
30.24 |
30.68 |
0.44 |
| 11 |
Aly-Archer, Kenzie N (7)
|
AMSTEE |
53.36 |
31.67 |
-21.69 |
| 12 |
Joyce, Honora G (7)
|
AM3RVS |
38.00 |
33.90 |
-4.10 |
| 13 |
Tomycz, Nolan G (7)
|
AMSTEE |
47.23 |
34.09 |
-13.14 |
| 14 |
McKinney, Sidney R (7)
|
AM3RVS |
38.00 |
37.60 |
-0.40 |
| 15 |
Coyle, Addison M (7)
|
AMSTEE |
54.25 |
41.04 |
-13.21 |
|
Chevganov, Maya (7)
|
AMPRA |
44.67 |
DQ |
- |
|
Schaupp, Liesl J (7)
|
AMNHA |
30.00 |
DQ |
- |
|
Rakow, Mila R (7)
|
AMNHA |
30.00 |
DQ |
- |
|
Kosky, Evelyn A (7)
|
AMNHA |
24.66 |
DQ |
- |
|
Wood, Tilda R (7)
|
AMNHA |
30.00 |
NS |
- |