| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cusworth, Tori G (11)
|
ADNTS |
34.38 |
34.05 |
-0.33 |
| 2 |
Braun, Julia L (12)
|
ADNTS |
33.97 |
35.12 |
1.15 |
| 3 |
McCarthy, Ella I (12)
|
ADNHA |
39.24 |
35.36 |
-3.88 |
| 4 |
Coupal, Mia A (11)
|
ADWAVE |
38.37 |
37.13 |
-1.24 |
| 5 |
Sekelj, Ella S (11)
|
ADNTS |
36.00 |
40.58 |
4.58 |
| 6 |
Eissenstat, Dilek S (12)
|
ADNTS |
45.15 |
43.95 |
-1.20 |
| 7 |
Heiner, Elizabeth A (12)
|
ADNHA |
51.09 |
44.88 |
-6.21 |
| 8 |
Shifrin, Yeva R (11)
|
ADNHA |
44.73 |
44.93 |
0.20 |
| 9 |
Collis, Isabella M (12)
|
ADNHA |
44.41 |
46.02 |
1.61 |
| 10 |
Christodoulou, Alexandra C (11)
|
ADWAVE |
47.22 |
48.07 |
0.85 |
| 11 |
Luley, Mya E (12)
|
ADNHA |
50.07 |
53.40 |
3.33 |
| 12 |
Simpson, Awemae G (11)
|
ADNTS |
|
1:29.79 |
- |
|
Jaros, Elaina N (11)
|
ADNHA |
50.49 |
DQ |
- |
|
Bashaw, Mikayla M (12)
|
ADNTS |
|
DQ |
- |
|
Mahapatra, Amirita V (11)
|
ADNTS |
1:02.20 |
NS |
- |