| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Murphy, Jenna (10)
|
OHHURY |
41.43 |
41.39 |
-0.04 |
| 2 |
McDonald, Lily P (10)
|
OHLTPY |
41.19 |
41.54 |
0.35 |
| 3 |
Scott, Caroline G (10)
|
OHLTPY |
47.72 |
41.88 |
-5.84 |
| 4 |
Dobozy, Libby J (10)
|
OHLTPY |
44.21 |
43.66 |
-0.55 |
| 5 |
Chan, Pallas * (10)
|
OHLTPY |
51.94 |
44.44 |
-7.50 |
| 6 |
Kelly, Alexa M (8)
|
OHLTPY |
|
47.09 |
- |
| 7 |
Pyles, Madison E (10)
|
OHHURY |
47.25 |
47.82 |
0.57 |
| 8 |
Bernard, Emily O (10)
|
OHLTPY |
50.22 |
47.90 |
-2.32 |
| 9 |
Nothem, Greta L (10)
|
OHHURY |
45.92 |
48.36 |
2.44 |
| 10 |
Wilkerson, Oliviah J (10)
|
OHLTPY |
45.75 |
49.18 |
3.43 |
| 11 |
Davis, Gwyneth A (9)
|
OHHURY |
45.47 |
52.35 |
6.88 |
| 12 |
Doty, Breck V (9)
|
OHDRY |
1:02.83 |
53.36 |
-9.47 |
| 13 |
Neerukonda, Anika S (10)
|
OHLTPY |
54.37 |
55.15 |
0.78 |
| 14 |
Radhakrishnan, Sumyuthka (10)
|
OHHURY |
54.94 |
56.37 |
1.43 |
| 15 |
Nothem, Mallory B (10)
|
OHHURY |
54.35 |
56.48 |
2.13 |
| 16 |
Hoffsis, Sophie S (6)
|
OHLTPY |
|
57.86 |
- |
| 17 |
Scharlott, EllaMae (9)
|
OHLTPY |
54.93 |
57.88 |
2.95 |
| 18 |
Griffith, Claire D (10)
|
OHHURY |
1:03.64 |
1:00.15 |
-3.49 |
| 19 |
Piggrem, Kadena L (8)
|
OHHURY |
1:01.72 |
1:00.42 |
-1.30 |
| 20 |
Boggs, Lilliana L (8)
|
OHHURY |
|
1:02.72 |
- |
| 21 |
Gerschutz, Lauren A (8)
|
OHHURY |
|
1:04.00 |
- |
| 22 |
Callif, Quinn R (9)
|
OHLTPY |
1:05.25 |
1:04.55 |
-0.70 |
| 23 |
Carey, Megan R (10)
|
OHDRY |
1:17.87 |
1:16.97 |
-0.90 |
|
Frank, Ava G (8)
|
OHHURY |
|
NS |
- |
|
Dokania, Arshiya (9)
|
OHHURY |
1:05.18 |
NS |
- |