| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ketler, Hannah J (10)
|
MDCAA |
41.71 |
35.90 |
-5.81 |
| 2 |
Lagana, Brooke A (10)
|
MDCAC |
39.04 |
35.91 |
-3.13 |
| 3 |
Tse, Grace E (9)
|
MDCAC |
|
38.74 |
- |
| 4 |
De La Cerda, Gabriela R (10)
|
MDCAC |
|
40.74 |
- |
| 5 |
Henchcliffe, Emma (9)
|
MDBAY |
34.76 |
40.79 |
6.03 |
| 6 |
Reconco, Zianni (10)
|
MDCAA |
47.39 |
43.32 |
-4.07 |
| 7 |
Mason-Mock, Sophia M (9)
|
MDCAC |
|
44.17 |
- |
| 8 |
Corelli, Kaitlyn (10)
|
MDCAC |
|
44.63 |
- |
| 9 |
Castillo, Alexandrina (10)
|
MDCAC |
|
45.21 |
- |
| 10 |
Stevens, Sydney A (9)
|
MDCAC |
|
45.29 |
- |
| 11 |
Kohl, Madeleine T (9)
|
MDCAC |
|
46.40 |
- |
| 12 |
Tanner, Tirzah E (9)
|
MDCAA |
41.01 |
46.58 |
5.57 |
| 13 |
Maldonado, Indigo S (10)
|
MDGMAC |
59.85 |
47.68 |
-12.17 |
| 14 |
Blackman, Maya R (9)
|
MDCAA |
43.71 |
49.20 |
5.49 |
| 15 |
Urban, Avery (10)
|
MDCAC |
|
49.35 |
- |
| 16 |
Ulmer, Grace S (9)
|
MDCAC |
|
49.81 |
- |
| 17 |
Thompson, Abigail M (10)
|
MDROCK |
1:00.03 |
53.74 |
-6.29 |
| 18 |
Pandit, Leela K (10)
|
MDCAA |
53.02 |
54.97 |
1.95 |