| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fulton, Alexa L (14)
|
MAUMLY |
30.89 |
28.99 |
-1.90 |
| 2 |
Pappas, Erini C (16)
|
MAUMLY |
29.55 |
29.73 |
0.18 |
| 3 |
Yuan, Allison M (17)
|
MAUMLY |
31.08 |
30.63 |
-0.45 |
| 4 |
Donnelly, Kiersten M (16)
|
MAUMLY |
30.02 |
31.26 |
1.24 |
| 5 |
Colizzo, Julia C (18)
|
MAUMLY |
32.25 |
31.35 |
-0.90 |
| 6 |
Lateef, Kazuri M (13)
|
NJMYM |
33.08 |
32.82 |
-0.26 |
| 7 |
Barton, Alison K (13)
|
NJMAY |
36.19 |
36.75 |
0.56 |
| 8 |
Posy, Sophia P (15)
|
NJMAY |
42.01 |
38.79 |
-3.22 |
| 9 |
Siow, Charlotte C (14)
|
NJMAY |
42.07 |
41.77 |
-0.30 |
| 10 |
Fredericks, Stormi M (13)
|
NJMAY |
44.58 |
43.54 |
-1.04 |