| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harrer, Sophia G (10)
|
NCUN |
3:55.47 |
3:10.95 |
-44.52 |
| 2 |
Napravnik, Sophia (10)
|
NCNCAC |
3:28.66 |
3:13.17 |
-15.49 |
| 3 |
Strunk, Julia A (9)
|
NCRSA |
|
3:36.58 |
- |
| 4 |
Taub, Anna J (10)
|
NCNCAC |
4:30.99 |
3:38.46 |
-52.53 |
| 5 |
Dever, Emerson B (10)
|
NCNCAC |
4:03.50 |
3:38.73 |
-24.77 |
| 6 |
Ciancanelli, Isabella R (9)
|
NCGFSC |
3:57.11 |
3:41.27 |
-15.84 |
| 7 |
Burton, Chloe G (9)
|
NCNCAC |
3:54.90 |
3:55.26 |
0.36 |
| 8 |
Hopkins, Alexis G (9)
|
NCMOR |
3:49.99 |
4:27.58 |
37.59 |
|
Jones, Phoebe E (10)
|
NCGFSC |
4:15.11 |
DQ |
- |
|
Keleher, Emma K (10)
|
NCGFSC |
3:34.67 |
DQ |
- |
|
Smithwick, Emerson C (9)
|
NCGFSC |
3:50.11 |
DQ |
- |
|
Brinkley, Elizabeth A (9)
|
NCMOR |
3:45.99 |
DQ |
- |
|
Cooper, Natalie M (10)
|
NCMOR |
4:00.93 |
DQ |
- |
|
Biswas, Emma A (9)
|
NCNCAC |
4:30.99 |
DQ |
- |
|
Martin, Annabelle E (9)
|
NCRSA |
|
DQ |
- |