| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Meyer, Penelope H (10)
|
ADDELM |
48.65 |
44.78 |
-3.87 |
| 2 |
Laurie, Josephine S (10)
|
ADHAWK |
55.00 |
48.41 |
-6.59 |
| 3 |
Wolfort, Madison J (10)
|
ADALBS |
55.71 |
49.22 |
-6.49 |
| 4 |
Cho, Anna Y (9)
|
ADALBS |
51.57 |
49.39 |
-2.18 |
| 5 |
Tirumala, Saanvi (10)
|
ADALBS |
53.36 |
51.03 |
-2.33 |
| 6 |
De Tuya, Elizabeth M (10)
|
ADDELM |
54.40 |
51.44 |
-2.96 |
| 7 |
Ziegler, Maggie S (10)
|
ADHAWK |
1:10.00 |
54.70 |
-15.30 |
| 8 |
Ilardi, Taylor E (10)
|
ADALBS |
53.79 |
56.32 |
2.53 |
| 9 |
Ho, Sophie (10)
|
ADDELM |
|
56.37 |
- |
| 10 |
Rajulu, Maegna K (10)
|
ADDELM |
1:18.01 |
57.01 |
-21.00 |
| 11 |
Williams, Isabella R (10)
|
ADALBS |
1:00.32 |
57.49 |
-2.83 |
| 12 |
Butler, Shayla G (10)
|
ADDELM |
58.88 |
57.60 |
-1.28 |
| 13 |
Villaume, Emma F (10)
|
ADALBS |
|
1:00.01 |
- |
| 14 |
Kane, Anna C (10)
|
ADDELM |
|
1:10.72 |
- |
|
Hebner, Charlotte G (10)
|
ADDELM |
|
DQ |
- |
|
Wolfort, Macy J (10)
|
ADALBS |
1:05.49 |
DQ |
- |
|
Cococcia, Mirabella A (9)
|
ADALBS |
|
DQ |
- |
|
Zhou, Olivia J (10)
|
ADALBS |
|
NS |
- |