| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brkanovic, Isak A (9)
|
ADDELM |
45.16 |
43.70 |
-1.46 |
| 2 |
Leung, Arthur (10)
|
ADUN |
|
45.66 |
- |
| 3 |
VanAlst, Amon J (10)
|
ADUN |
46.96 |
48.27 |
1.31 |
| 4 |
Killian, Daniel S (10)
|
ADCPP |
49.34 |
49.66 |
0.32 |
| 5 |
Cao, James Y (9)
|
ADDELM |
54.05 |
50.85 |
-3.20 |
| 6 |
Staudinger, Samuel P (9)
|
ADALBS |
53.29 |
51.83 |
-1.46 |
| 7 |
Witting, Liam T (9)
|
ADDELM |
59.69 |
51.99 |
-7.70 |
| 8 |
Simmons, Cooper Z (10)
|
ADCPP |
51.88 |
52.42 |
0.54 |
| 9 |
Wang, Alexander (9)
|
ADDELM |
1:01.69 |
53.17 |
-8.52 |
| 10 |
Hook, Gavin M (10)
|
ADCPP |
56.12 |
53.95 |
-2.17 |
|
LaFountain, Jackson P (9)
|
ADALBS |
|
DQ |
- |
|
LaFountain, Tobias P (10)
|
ADALBS |
1:21.52 |
DQ |
- |
|
Keller, Brady C (10)
|
ADCPP |
1:02.44 |
DQ |
- |
|
Upton, Matthew T (10)
|
ADCPP |
1:04.20 |
DQ |
- |
|
Nabaoui, Abdullah (9)
|
ADCPP |
|
NS |
- |