| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gibson, Alexis M (10)
|
ADALBS |
35.33 |
33.91 |
-1.42 |
| 2 |
Ramgulam, Varshana (10)
|
ADCPP |
38.18 |
35.56 |
-2.62 |
| 3 |
Williams, Sarah L (10)
|
ADDELM |
|
39.62 |
- |
| 4 |
Sands, Samantha B (10)
|
ADCPP |
|
39.79 |
- |
| 5 |
Stoup, Anna L (9)
|
ADCPP |
36.54 |
39.81 |
3.27 |
| 6 |
Antolick, Caroline B (10)
|
ADALBS |
39.02 |
40.93 |
1.91 |
| 7 |
Michels, Delaney R (10)
|
ADCPP |
39.34 |
41.81 |
2.47 |
| 8 |
Zhang, Stella Y (9)
|
ADCPP |
41.71 |
42.32 |
0.61 |
| 9 |
Silaika, Genevieve E (9)
|
ADCPP |
46.71 |
43.46 |
-3.25 |
| 10 |
Hong, Allison (9)
|
ADCPP |
44.58 |
44.92 |
0.34 |
| 11 |
Dube, Ava C (9)
|
ADALBS |
44.96 |
45.14 |
0.18 |
| 12 |
Becker, Serinda C (9)
|
ADALBS |
52.47 |
45.27 |
-7.20 |
| 13 |
Liang, Olivia Y (10)
|
ADALBS |
52.04 |
45.35 |
-6.69 |
| 14 |
Domitrovits, Charlee E (10)
|
ADHAWK |
43.89 |
45.40 |
1.51 |
| 15 |
Xue, Nichole Y (9)
|
ADCPP |
44.19 |
46.65 |
2.46 |
| 16 |
Conklin, Charlotte E (10)
|
ADCPP |
47.13 |
46.73 |
-0.40 |
| 17 |
Kopiec, Ashley R (10)
|
NESNAP |
47.97 |
46.85 |
-1.12 |
| 18 |
Vaccaro, Lauren C (9)
|
ADALBS |
|
48.09 |
- |
| 19 |
Bieniek, Allison M (9)
|
ADCPP |
48.58 |
48.30 |
-0.28 |
| 20 |
Jiao, Ava (10)
|
ADALBS |
|
48.58 |
- |
| 21 |
Suchowiecki, Kate M (9)
|
ADHAWK |
49.10 |
48.68 |
-0.42 |
| 22 |
Cho, Eileen Y (10)
|
ADALBS |
49.23 |
52.39 |
3.16 |
| 23 |
Carsillo, Gianna I (10)
|
ADDELM |
|
53.67 |
- |
| 24 |
Chavali, Thanya (9)
|
ADALBS |
56.44 |
54.61 |
-1.83 |
| 25 |
Skuza, Olivia (10)
|
ADDELM |
|
54.87 |
- |
| 26 |
Tripp, Donja A (9)
|
ADALBS |
54.51 |
55.02 |
0.51 |
| 27 |
Sellnow, Emma M (9)
|
ADEELS |
|
58.72 |
- |
| 28 |
Deitz, Makenna R (10)
|
ADALBS |
|
1:00.57 |
- |
|
Fabregas, Selma A (9)
|
ADDELM |
|
DQ |
- |