| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wong, Preston S (11)
|
VANOVA |
34.90 |
33.46 |
-1.44 |
| 2 |
Bachrach, Jacob E (11)
|
PVNCAP |
34.91 |
34.45 |
-0.46 |
| 3 |
Mikhailov-Bakker, Dmitry J (11)
|
PVNCAP |
35.78 |
34.49 |
-1.29 |
| 4 |
Linkonis, Richard P (11)
|
VANOVA |
34.49 |
34.61 |
0.12 |
| 5 |
Chethan, Ayan (11)
|
PVNCAP |
36.06 |
34.95 |
-1.11 |
| 6 |
Quach, Andrew (11)
|
MRLIAC |
36.17 |
36.00 |
-0.17 |
| 7 |
Harrington, Luke P (11)
|
VANOVA |
38.03 |
36.89 |
-1.14 |
| 8 |
Cornelius, Xavier M (11)
|
VANOVA |
39.49 |
38.16 |
-1.33 |
| 9 |
Corsino, Jaden Lucas E (11)
|
PVNCAP |
37.44 |
38.29 |
0.85 |
| 10 |
Allison, John G (11)
|
VATIDE |
38.46 |
38.36 |
-0.10 |
| 11 |
Oluwabusola, Ean A (11)
|
VATIDE |
39.24 |
38.80 |
-0.44 |
| 12 |
Bennett, James C (11)
|
VATIDE |
40.79 |
40.35 |
-0.44 |
| 13 |
Diamantis, Evangelos N (11)
|
MRLIAC |
46.65 |
41.30 |
-5.35 |
| 14 |
Nguyen, James R (11)
|
MRLIAC |
43.80 |
43.62 |
-0.18 |
| 15 |
Morrozoff, Silas S (11)
|
VATIDE |
49.00 |
50.67 |
1.67 |