| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morgan, Alexandra M (12)
|
PVNCAP |
34.66 |
32.21 |
-2.45 |
| 2 |
Chen, Bella S (12)
|
PVNCAP |
34.64 |
34.06 |
-0.58 |
| 3 |
Dufault, Audrey A (12)
|
PVNCAP |
33.44 |
34.20 |
0.76 |
| 4 |
Banks, Hadley (12)
|
VANOVA |
34.96 |
34.22 |
-0.74 |
| 5 |
Bouchard, Gabrielle M (12)
|
VANOVA |
35.82 |
34.62 |
-1.20 |
| 6 |
Randsmith, Saxon K (12)
|
PVNCAP |
35.50 |
34.73 |
-0.77 |
| 7 |
Peterson, Campbell M (12)
|
VATIDE |
35.97 |
35.20 |
-0.77 |
| 8 |
Blackston, Anna M (12)
|
VANOVA |
37.94 |
35.75 |
-2.19 |
| 9 |
Goodloe, Luna S (12)
|
VATIDE |
35.05 |
35.96 |
0.91 |
| 10 |
Shean, May E (12)
|
VATIDE |
36.77 |
36.86 |
0.09 |
| 11 |
Gans, Josie L (12)
|
MRLIAC |
39.29 |
36.95 |
-2.34 |
| 12 |
Hudson, Ella J (12)
|
VANOVA |
36.91 |
36.98 |
0.07 |
| 13 |
Hu, Allison (12)
|
MRLIAC |
38.72 |
37.46 |
-1.26 |
| 14 |
Piper, Vanessa L (12)
|
VATIDE |
39.79 |
38.49 |
-1.30 |