| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tang, Kai Ting (12)
|
MRLIAC |
24.62 |
24.78 |
0.16 |
| 2 |
Causey, Charlotte E (12)
|
VANOVA |
26.13 |
26.30 |
0.17 |
| 3 |
Trinh, Ellie M (12)
|
PVNCAP |
27.11 |
26.34 |
-0.77 |
| 4 |
Munchel, Dylan R (12)
|
PVNCAP |
26.71 |
26.47 |
-0.24 |
| 5 |
Taylor, Olivia L (12)
|
VATIDE |
26.51 |
26.49 |
-0.02 |
| 6 |
Hull, Elliana G (12)
|
VANOVA |
27.41 |
26.52 |
-0.89 |
| 7 |
Davy-Brown, Layla S (12)
|
PVNCAP |
27.11 |
26.93 |
-0.18 |
| 8 |
Gavino, Alessandra B (12)
|
PVNCAP |
27.64 |
27.01 |
-0.63 |
| 9 |
Dee, Tamara Z (12)
|
VANOVA |
28.56 |
28.39 |
-0.17 |
| 9 |
Hight, Katherine L (12)
|
VANOVA |
28.29 |
28.39 |
0.10 |
| 11 |
Fickenscher, Margaret A (12)
|
VATIDE |
29.80 |
28.73 |
-1.07 |
| 12 |
Wilson, Loralei E (12)
|
VATIDE |
29.11 |
29.05 |
-0.06 |
| 13 |
Wilkerson, Charlotte A (12)
|
VATIDE |
30.38 |
29.98 |
-0.40 |