| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baldwin, Ryan T (13)
|
MRLIAC |
53.29 |
52.08 |
-1.21 |
| 2 |
Muminovic, Aidin A (13)
|
VANOVA |
54.54 |
52.67 |
-1.87 |
| 3 |
Cortez Martinez, Adrian A (13)
|
PVNCAP |
57.15 |
58.25 |
1.10 |
| 4 |
Harrington, Charlie W (13)
|
VANOVA |
59.67 |
58.29 |
-1.38 |
| 5 |
Miceli, Jack M (13)
|
PVNCAP |
58.74 |
58.52 |
-0.22 |
| 6 |
Valentine, Jack M (13)
|
VATIDE |
1:01.73 |
1:00.23 |
-1.50 |
| 7 |
Pan, Victor C (13)
|
MRLIAC |
1:00.47 |
1:00.31 |
-0.16 |
| 8 |
Jacobs, Benjamin P (13)
|
PVNCAP |
1:01.96 |
1:00.51 |
-1.45 |
| 9 |
Hartanto, Alexander (13)
|
PVNCAP |
1:01.75 |
1:01.02 |
-0.73 |
| 10 |
Harrington, Cullen S (13)
|
VANOVA |
1:00.95 |
1:01.06 |
0.11 |
| 11 |
Ricotta, Steven A (13)
|
MRLIAC |
1:02.67 |
1:01.34 |
-1.33 |
| 12 |
Leach, Matthew A (13)
|
VATIDE |
1:03.49 |
1:02.03 |
-1.46 |
| 13 |
Rivet, James B (13)
|
VANOVA |
1:03.38 |
1:02.21 |
-1.17 |
| 14 |
Filtser Melendez, Ian V (13)
|
MRLIAC |
1:03.64 |
1:02.63 |
-1.01 |
| 15 |
Council, Jaedan Z (13)
|
VATIDE |
1:09.62 |
1:07.59 |
-2.03 |
| 16 |
Wood, Wilson (13)
|
VATIDE |
1:08.05 |
1:11.54 |
3.49 |