| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Abbott, Trent M (10)
|
VANOVA |
31.46 |
30.59 |
-0.87 |
| 2 |
Lyashenko, Christopher V (10)
|
VATIDE |
31.73 |
31.00 |
-0.73 |
| 3 |
Li, Kingston C (10)
|
MRLIAC |
32.93 |
31.39 |
-1.54 |
| 4 |
Meehan, Luke P (10)
|
MRLIAC |
34.77 |
32.52 |
-2.25 |
| 5 |
Ferrigno, Riccardo (10)
|
VATIDE |
34.79 |
33.40 |
-1.39 |
| 6 |
Burns, William A (10)
|
VANOVA |
36.85 |
34.36 |
-2.49 |
| 7 |
Hall, David A (10)
|
PVNCAP |
35.97 |
34.45 |
-1.52 |
| 8 |
Soundappan, Jayden P (10)
|
PVNCAP |
34.87 |
34.49 |
-0.38 |
| 9 |
Okolo, John-Matthew O (10)
|
PVNCAP |
35.33 |
34.80 |
-0.53 |
| 10 |
Rall, Preston J (10)
|
PVNCAP |
35.34 |
35.17 |
-0.17 |
| 11 |
Sunkavalli, Adhvik (10)
|
VANOVA |
36.80 |
35.54 |
-1.26 |
| 12 |
Schaffter, Rylan A (10)
|
VATIDE |
37.88 |
38.75 |
0.87 |
| 13 |
Greer, Ian J (10)
|
VANOVA |
|
40.91 |
- |
| 14 |
Kolpakov, Andrew Y (10)
|
VATIDE |
45.66 |
44.37 |
-1.29 |