| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vernon, Allison L (13)
|
VAQSTS |
54.63 |
54.93 |
0.30 |
| 2 |
Canada, Raegan C (13)
|
VAQSTS |
54.59 |
55.37 |
0.78 |
| 3 |
Cosner, Emerson G (14)
|
VAQSTS |
55.30 |
56.52 |
1.22 |
| 4 |
Linkonis, Gabriela M (13)
|
VANOVA |
57.11 |
57.75 |
0.64 |
| 5 |
Sivak, Audrey L (14)
|
VAQSTS |
58.16 |
58.20 |
0.04 |
| 6 |
Baraceros, Kamille D (14)
|
VAQSTS |
57.08 |
58.28 |
1.20 |
| 6 |
Diggs, Ellen C (13)
|
VAQSTS |
57.82 |
58.28 |
0.46 |
| 8 |
Phillips, Elena L (14)
|
VAQSTS |
56.66 |
59.57 |
2.91 |
| 9 |
Bryant, Maeve K (14)
|
VAQSTS |
59.84 |
1:00.11 |
0.27 |
| 10 |
Springer, Gabriella M (14)
|
VAQSTS |
59.79 |
1:00.50 |
0.71 |
| 11 |
Maksimovic, Sara (14)
|
VANOVA |
1:00.66 |
1:00.67 |
0.01 |
| 12 |
Ix, Sara D (14)
|
VAQSTS |
1:00.15 |
1:01.26 |
1.11 |
| 13 |
Lundeberg, Ilsa W (14)
|
VAQSTS |
58.97 |
1:01.65 |
2.68 |
|
Reker, Ava V (13)
|
VAQSTS |
59.57 |
NS |
- |