| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Karasiewicz, Hayden A (7)
|
MDBAY |
38.62 |
38.64 |
0.02 |
| 2 |
Nolde, Anna E (8)
|
VAACAC |
37.75 |
40.85 |
3.10 |
| 3 |
Goff, Emmalyn G (8)
|
VACGBD |
45.03 |
43.70 |
-1.33 |
| 4 |
Raeburn, Morgan Delaney (7)
|
VACVAC |
43.57 |
43.77 |
0.20 |
| 5 |
Baldwin, Natalie Q (7)
|
VASRVA |
40.94 |
43.86 |
2.92 |
| 6 |
Schwarzenberg-Word, Alia (8)
|
VACGBD |
45.95 |
47.65 |
1.70 |
| 7 |
Pitts, Caitlin W (8)
|
MDBAY |
|
48.88 |
- |
| 8 |
Lewis, Reagan Q (8)
|
MDBAY |
57.89 |
52.97 |
-4.92 |
| 9 |
Greer, Ashante L (7)
|
VASRVA |
54.18 |
53.24 |
-0.94 |
| 10 |
Meledick, Hannah R (7)
|
MDBAY |
1:01.60 |
54.63 |
-6.97 |
| 11 |
Shipe, Chloe E (8)
|
VACGBD |
55.49 |
54.96 |
-0.53 |
| 12 |
Quick, Leila K (8)
|
VACVAC |
1:01.86 |
1:00.90 |
-0.96 |