| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ismailova, Diana (8)
|
VAGATR |
37.63 |
36.86 |
-0.77 |
| 2 |
Beyer, Victoria S (8)
|
VASTAT |
|
47.84 |
- |
| 2 |
Gorby, Lila (7)
|
VAGATR |
48.71 |
47.84 |
-0.87 |
| 4 |
Robertson, Catherine L (8)
|
VAPSDN |
50.56 |
48.56 |
-2.00 |
| 5 |
Pew, Ashlyn G (8)
|
VAPSDN |
49.78 |
49.44 |
-0.34 |
| 6 |
Suttell, Adrianna F (8)
|
VAGATR |
|
51.03 |
- |
| 7 |
Adams, Jordan R (7)
|
VAPSDN |
|
53.02 |
- |
| 8 |
Galang, Katherine A (8)
|
VAPSDN |
52.27 |
53.84 |
1.57 |
| 9 |
Pew, Isabella F (7)
|
VAPSDN |
54.13 |
54.83 |
0.70 |
| 10 |
Callihan, Catherine B (8)
|
VAPSDN |
54.76 |
1:00.52 |
5.76 |
| 11 |
Sandlin, Ashlyn M (8)
|
VAPSDN |
|
1:00.64 |
- |
| 12 |
Carreras, Kathryn (8)
|
VAPSDN |
1:10.62 |
1:06.00 |
-4.62 |
|
Dugger, Sophia A (7)
|
VADC |
|
DQ |
- |