| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ismailova, Diana (9)
|
VAGATR |
31.74 |
30.52 |
-1.22 |
| 2 |
Kestner, Lee R (10)
|
VAGATR |
31.92 |
32.61 |
0.69 |
| 3 |
Salerno, Isabella M (10)
|
VAGATR |
32.98 |
32.74 |
-0.24 |
| 4 |
Gorby, Tessa L (10)
|
VAGATR |
32.94 |
32.86 |
-0.08 |
| 5 |
Bradberry, Abigail L (10)
|
VAGATR |
33.17 |
33.11 |
-0.06 |
| 6 |
Sylvester-Johnson, Jillian B (9)
|
VAGATR |
34.80 |
33.49 |
-1.31 |
| 7 |
Wells, Sara T (10)
|
VAGATR |
36.12 |
33.95 |
-2.17 |
| 8 |
Czirjak, Sara E (10)
|
VAGATR |
35.90 |
34.65 |
-1.25 |
| 9 |
Murtaugh, Lindsay E (9)
|
VAGATR |
34.20 |
34.96 |
0.76 |
| 10 |
Moody, Macy H (10)
|
VAGATR |
34.18 |
35.20 |
1.02 |
| 11 |
Keller, Madison G (10)
|
VAGATR |
41.72 |
35.24 |
-6.48 |
| 12 |
Sofi, Alishba (8)
|
VAGATR |
37.14 |
35.86 |
-1.28 |
| 13 |
Schaaf, Alexis V (9)
|
VAGATR |
37.98 |
36.20 |
-1.78 |
| 14 |
Moseley, Kaitlyn S (9)
|
VAGATR |
47.80 |
46.82 |
-0.98 |
| 15 |
Shuler, Ella M (10)
|
VASCAT |
43.63 |
47.20 |
3.57 |
| 16 |
Jones, Cary E (9)
|
VASCAT |
45.55 |
52.30 |
6.75 |