| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Skinner, Jack W (8)
|
VAHOKI |
49.88 |
48.32 |
-1.56 |
| 2 |
Ayers, Tilden D (8)
|
VACCA |
47.65 |
49.52 |
1.87 |
| 3 |
Hottle, Luke M (8)
|
VAGATR |
52.01 |
50.07 |
-1.94 |
| 4 |
Shelton-Barnes, Tyus J (8)
|
VAACAC |
53.07 |
52.91 |
-0.16 |
| 5 |
Tungate, Benjamin L (8)
|
VACYAC |
55.04 |
52.94 |
-2.10 |
| 6 |
Johnson, James C (8)
|
VABASS |
56.67 |
54.03 |
-2.64 |
| 7 |
Cullaty, John O (8)
|
VAGATR |
56.61 |
54.97 |
-1.64 |
| 8 |
Starr, Bryson S (7)
|
VAHOKI |
54.96 |
56.28 |
1.32 |
| 9 |
Giovannini, Niccolo J (7)
|
VACCA |
|
56.56 |
- |
| 10 |
Thompson, Isaiah J (8)
|
VAACAC |
|
56.93 |
- |
| 11 |
Easton, Walker C (6)
|
VAACAC |
|
58.45 |
- |
| 12 |
Gross, Miller T (8)
|
VACCA |
58.54 |
59.68 |
1.14 |
|
Christiansen, Kemper M (6)
|
VACCA |
|
DQ |
- |
|
Madan, Aryan (7)
|
VACCA |
|
DQ |
- |
|
Phlipot, Jonathan J (7)
|
VACCA |
1:11.61 |
DQ |
- |
|
Yardley, Wynn K (7)
|
VACCA |
|
DQ |
- |
|
Harrington, Landon (6)
|
VALY |
|
DQ |
- |
|
Tungate, Maxwell L (6)
|
VACYAC |
|
DQ |
- |
|
Xu, Evan (8)
|
VACYAC |
|
DQ |
- |