| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Frenzel, Jack W (10)
|
NIWEBS |
38.04 |
36.37 |
-1.67 |
| 2 |
Weber, Joshua P (10)
|
NIWEBS |
36.87 |
36.80 |
-0.07 |
| 3 |
Rene, Jax R (10)
|
NIIRON |
39.11 |
37.38 |
-1.73 |
| 4 |
Ernst, Emmett J (10)
|
NIWEBS |
38.67 |
39.13 |
0.46 |
| 5 |
Barnhart, Nicolas D (9)
|
NIWEBS |
43.54 |
43.13 |
-0.41 |
| 6 |
Kumar, Dwaraka S (9)
|
NIWEBS |
44.38 |
47.88 |
3.50 |
| 7 |
Steele, Thomas J (9)
|
NIWEBS |
45.16 |
48.72 |
3.56 |
| 8 |
Pellman, Jonathan P (9)
|
NIIRON |
50.29 |
49.10 |
-1.19 |
| 9 |
Giarrizzohoyle, Joseph B (10)
|
NIWEBS |
|
49.85 |
- |
| 10 |
Giarrizzohoyle, John A (10)
|
NIWEBS |
|
51.43 |
- |
| 11 |
Madison, Owen P (10)
|
NIWEBS |
53.71 |
52.47 |
-1.24 |