| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stahl, Reed P (10)
|
NIVICT |
45.36 |
43.44 |
-1.92 |
| 2 |
O'Dea, Daniel W (10)
|
NIGRSC |
46.21 |
43.73 |
-2.48 |
| 3 |
Dinh, Brendan D (10)
|
NIWEBS |
47.51 |
44.79 |
-2.72 |
| 4 |
Cabral, Carlton (10)
|
NIRRST |
57.15 |
46.38 |
-10.77 |
| 5 |
Stancampiano, Neel M (10)
|
NIPACK |
47.58 |
48.03 |
0.45 |
| 6 |
Gonzalez, Charles M (10)
|
NIPACK |
48.23 |
48.17 |
-0.06 |
| 7 |
Ray, Gavin M (10)
|
NIVICT |
46.49 |
49.13 |
2.64 |
| 8 |
Richards-Dyson, Jax E (10)
|
NIGCOM |
52.18 |
50.91 |
-1.27 |
| 9 |
Carlton, Caleb D (10)
|
NIWEBS |
55.11 |
54.47 |
-0.64 |
| 10 |
Schmidt, Millenium K (10)
|
NIRRST |
47.65 |
57.09 |
9.44 |
| 11 |
Panfil, Tyler J (10)
|
NIGRSC |
1:00.46 |
57.11 |
-3.35 |
| 12 |
Shields, Zachary W (10)
|
NIVICT |
55.40 |
1:02.08 |
6.68 |
| 13 |
Madison, Benjamin M (10)
|
NIWEBS |
|
1:09.96 |
- |
| 14 |
He, Andrew (10)
|
NIVICT |
1:10.67 |
1:11.76 |
1.09 |
|
Schmidt, Million K (10)
|
NIRRST |
|
DQ |
- |
|
Melendez, Ezekiel I (10)
|
NIRRST |
|
NS |
- |