| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bouwens, Emma L (11)
|
NIHAST |
37.20 |
34.92 |
-2.28 |
| 2 |
Robinson, Sara C (11)
|
NISDSC |
38.04 |
36.28 |
-1.76 |
| 3 |
Share, Elizabeth A (11)
|
NIPMSC |
42.51 |
36.41 |
-6.10 |
| 4 |
Camnitz, Anna (11)
|
NIUN-W |
40.87 |
40.58 |
-0.29 |
| 5 |
Taylor, Janey A (11)
|
NIVICT |
42.97 |
42.75 |
-0.22 |
| 6 |
Smolinski, Evelynn J (11)
|
NIHAST |
45.83 |
43.93 |
-1.90 |
| 7 |
Kuhlkin, Elizabeth C (11)
|
NIGCOM |
49.30 |
45.03 |
-4.27 |
| 8 |
Resch, Rebecka J (11)
|
NIGRSC |
46.99 |
48.04 |
1.05 |
| 9 |
Thurston, Lauren A (11)
|
NIGCOM |
50.10 |
50.09 |
-0.01 |
| 10 |
Rist, Olivia B (11)
|
NIHAST |
1:22.82 |
51.84 |
-30.98 |
| 11 |
Farina, Samantha R (11)
|
NIGRSC |
56.40 |
55.25 |
-1.15 |
| 12 |
Greisch, Alisha D (11)
|
NIGRSC |
56.49 |
58.52 |
2.03 |