| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mettu, Pranav R (12)
|
NCRMY |
33.47 |
31.31 |
-2.16 |
| 2 |
Beaman, Matthew H (11)
|
NCAQUA |
32.56 |
31.72 |
-0.84 |
| 3 |
Allis, Clark E (11)
|
NCAQUA |
31.60 |
32.24 |
0.64 |
| 4 |
Mitchell, Carter O (11)
|
NCAQUA |
33.61 |
32.56 |
-1.05 |
| 5 |
Wozniak, Dominic I (11)
|
NCRMY |
36.49 |
33.25 |
-3.24 |
| 6 |
Kucharczyk, Braiden J (11)
|
NCASTF |
34.68 |
33.41 |
-1.27 |
| 7 |
Allis, Elijah C (11)
|
NCAQUA |
35.28 |
35.46 |
0.18 |
| 8 |
Crumley, Samuel B (11)
|
NCRMY |
36.30 |
35.84 |
-0.46 |
| 9 |
Magsino, Dwayne Zachary C (12)
|
NCRMY |
37.13 |
36.34 |
-0.79 |
| 10 |
Burnworth, Walton C (11)
|
NCWBST |
|
37.62 |
- |
| 11 |
Quinn, Trathen D (12)
|
NCAQUA |
|
38.80 |
- |
| 12 |
Honeycutt, Joseph C (11)
|
NCVACB |
44.92 |
46.14 |
1.22 |
|
Burns, Kaden A (11)
|
NCFISH |
47.25 |
NS |
- |