| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Torsone, Bryce G (9)
|
NCGFSC |
45.73 |
44.81 |
-0.92 |
| 2 |
Delos Santos, Joshua B (8)
|
NCGFSC |
47.88 |
46.16 |
-1.72 |
| 3 |
Johnson, Connor R (10)
|
NCGOLD |
50.86 |
47.85 |
-3.01 |
| 4 |
Mettu, Pranav R (9)
|
NCRMY |
1:21.02 |
48.21 |
-32.81 |
| 5 |
Earl, Tyler K (10)
|
UNAT |
45.97 |
50.64 |
4.67 |
| 6 |
Clayton, Thomas J (9)
|
NCGFSC |
53.17 |
50.70 |
-2.47 |
| 7 |
Aberant, Thomas A (10)
|
NCGFSC |
52.42 |
52.53 |
0.11 |
| 8 |
Pendleton, Zackary A (10)
|
NCGFSC |
53.74 |
53.97 |
0.23 |
| 9 |
Singletary, Cohen P (10)
|
NCLTNC |
52.99 |
54.46 |
1.47 |
| 10 |
Payne, Charlie W (9)
|
NCGFSC |
59.26 |
58.23 |
-1.03 |
| 11 |
Prakash, Anirudh (9)
|
NCWBST |
1:09.53 |
59.08 |
-10.45 |
| 12 |
Gribbin, Harry G (9)
|
NCGOLD |
59.09 |
1:00.10 |
1.01 |
| 13 |
Lee, Christopher W (9)
|
NCRMY |
|
1:03.17 |
- |
| 14 |
Jones, Noah C (9)
|
NCYBAC |
1:05.09 |
1:03.79 |
-1.30 |
| 15 |
Rodeffer, Matthew J (9)
|
NCLTNC |
|
1:09.71 |
- |
|
Coetzer, Eli C (10)
|
NCWBST |
|
DQ |
- |