| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhang, Michael G (17)
|
PVFISH |
|
21.87 |
- |
| 2 |
Hendrickson, Andrew D (16)
|
PVNCAP |
22.41 |
22.29 |
-0.12 |
| 3 |
Schmid, JT (15)
|
PVNCAP |
24.00 |
22.44 |
-1.56 |
| 4 |
Rustia, Tomas M (18)
|
PVNCAP |
23.61 |
23.45 |
-0.16 |
| 5 |
Popovich, Cole P (15)
|
PVFISH |
23.78 |
23.72 |
-0.06 |
| 6 |
Adams, Grant S (15)
|
PVNCAP |
22.90 |
24.12 |
1.22 |
| 7 |
Tain, Connor P (14)
|
PVNCAP |
24.82 |
24.21 |
-0.61 |
| 8 |
Gorman, Dax P (16)
|
PVNCAP |
25.07 |
24.39 |
-0.68 |
| 9 |
Goggins, Christopher D (15)
|
PVNCAP |
27.19 |
24.63 |
-2.56 |
| 10 |
Wyszogrodzki, Wiktor (14)
|
PVFISH |
25.10 |
24.75 |
-0.35 |
| 11 |
Ly, Nicholas M (16)
|
PVNCAP |
25.06 |
24.81 |
-0.25 |
| 12 |
Martin, Allison M (18)
|
PVFISH |
24.02 |
24.86 |
0.84 |
| 13 |
Murray, Meghan E (16)
|
PVFISH |
25.07 |
25.18 |
0.11 |
| 14 |
Redman, Emma L (14)
|
PVFISH |
25.21 |
25.64 |
0.43 |
| 15 |
Wargo, Laura M (16)
|
UNAT |
24.92 |
25.68 |
0.76 |
| 16 |
Srun, Tidawee V (17)
|
PVNCAP |
26.25 |
26.26 |
0.01 |
| 17 |
Lewis, Sydney C (15)
|
PVFISH |
26.45 |
26.31 |
-0.14 |
| 18 |
Charles, Makayla L (17)
|
PVNCAP |
25.00 |
26.52 |
1.52 |
| 19 |
Van Beek, Skye T (17)
|
PVNCAP |
26.92 |
26.66 |
-0.26 |