| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ramey, Kenna N (18)
|
PNKING |
|
26.23 |
- |
| 2 |
Taylor, Danielle M (17)
|
PCWCAB |
27.20 |
26.70 |
-0.50 |
| 3 |
Hynes, Haley M (17)
|
MVESC |
26.32 |
26.88 |
0.56 |
| 4 |
Koontz, Shelby L (17)
|
PCDDST |
27.10 |
27.10 |
0.00 |
| 5 |
Aycock, Courtney T (18)
|
SETNAQ |
26.94 |
27.19 |
0.25 |
| 6 |
Kudis, Danielle B (18)
|
WISSTY |
27.90 |
27.30 |
-0.60 |
| 7 |
Hanas, Andrea R (16)
|
INDUNE |
27.57 |
27.49 |
-0.08 |
| 8 |
Souther, Alexis D (16)
|
NCMAC |
27.35 |
27.54 |
0.19 |
| 8 |
Schillinger, Marie-Claire (18)
|
PCWCAB |
28.42 |
27.54 |
-0.88 |
| 10 |
Korenwinder, Mallory A (17)
|
CCTNT |
27.96 |
27.58 |
-0.38 |
| 11 |
Cole, Leslie M (18)
|
PVNCAP |
27.98 |
28.08 |
0.10 |
|
Matheson, Eleanor G (16)
|
COFAC |
26.26 |
NS |
- |