| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolfort, Madison J (14)
|
ADALBS |
5:56.06 |
5:41.48 |
-14.58 |
| 2 |
Dunbar, May (14)
|
ADUSAM |
5:59.28 |
5:46.76 |
-12.52 |
| 3 |
Bryant, Mia V (14)
|
ADDELM |
6:10.04 |
5:54.38 |
-15.66 |
| 4 |
Cordick, Helena E (14)
|
ADWAVE |
5:53.95 |
5:55.54 |
1.59 |
| 5 |
Jennings, Ava G (14)
|
ADSCHE |
5:54.81 |
5:55.89 |
1.08 |
| 6 |
Cunningham, Isabelle G (13)
|
ADCPP |
6:03.85 |
6:00.76 |
-3.09 |
| 7 |
Newcomb, Lucy G (14)
|
ADHAWK |
6:07.20 |
6:02.38 |
-4.82 |
| 8 |
Rio, Willa K (13)
|
ADNHA |
6:06.41 |
6:02.78 |
-3.63 |
| 9 |
Deal, Grace A (14)
|
ADCLIN |
6:05.05 |
6:02.89 |
-2.16 |
| 10 |
Breckenridge, Lucy F (13)
|
ADSCHE |
6:03.12 |
6:03.00 |
-0.12 |
| 11 |
Stoup, Madelyn L (14)
|
ADCPP |
6:00.53 |
6:04.48 |
3.95 |
| 12 |
Relyea, Cadence G (13)
|
ADDELM |
6:06.43 |
6:05.00 |
-1.43 |
| 13 |
Forstell, Abigail D (13)
|
ADHAWK |
6:02.57 |
6:05.54 |
2.97 |
| 14 |
Lee, Olivia C (13)
|
ADALBS |
6:19.27 |
6:09.20 |
-10.07 |
| 15 |
Carroll, Sara J (13)
|
ADDELM |
6:13.72 |
6:10.27 |
-3.45 |
| 16 |
Ventura, Colbie A (14)
|
ADSCHE |
6:18.44 |
6:16.60 |
-1.84 |
| 17 |
Toomajian, Stella T (13)
|
ADUSAM |
6:25.61 |
6:27.53 |
1.92 |
| 18 |
Gloak, Gemma M (14)
|
ADUSAM |
6:25.83 |
6:32.16 |
6.33 |