| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stafford, Keegan D (17)
|
ADSCHE |
48.69 |
49.24 |
0.55 |
| 2 |
Pokines, Addison J (16)
|
ADSCHE |
51.80 |
50.96 |
-0.84 |
| 3 |
Karaca, Rowan H (13)
|
ADSCHE |
58.92 |
55.85 |
-3.07 |
| 4 |
Sellnow, Abigail V (15)
|
ADUN |
55.43 |
57.32 |
1.89 |
| 5 |
Versaci, Rosemarie A (17)
|
ADSCHE |
55.50 |
57.43 |
1.93 |
| 6 |
Nelson, Isabel M (16)
|
ADSCHE |
54.07 |
57.53 |
3.46 |
| 7 |
O'Donnell, Maura E (14)
|
ADUN |
57.35 |
57.66 |
0.31 |
| 8 |
Felix, Morgan A (17)
|
ADSCHE |
|
58.91 |
- |
| 9 |
Gregory, John D (13)
|
ADSCHE |
1:02.24 |
1:00.74 |
-1.50 |
| 10 |
Bogdanowicz-Wilson, Emma K (16)
|
ADSCHE |
58.00 |
1:01.11 |
3.11 |
| 11 |
O'Leary, Anthony Y (13)
|
ADSCHE |
1:05.29 |
1:02.97 |
-2.32 |
| 12 |
Splendido, Luke J (13)
|
ADSCHE |
1:10.38 |
1:06.04 |
-4.34 |