| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reubi, Natalie R (12)
|
AMANSC |
2:39.15 |
2:37.97 |
-1.18 |
| 2 |
Motley, Eleanor R (11)
|
AMSTEE |
2:56.41 |
2:39.60 |
-16.81 |
| 3 |
Fisher, Carrie J (11)
|
AMSTEE |
2:59.47 |
2:51.16 |
-8.31 |
| 4 |
Murray, Kate (12)
|
AMBYAC |
2:57.39 |
2:53.84 |
-3.55 |
| 5 |
Crowley, Thea L (11)
|
AMNHA |
3:05.66 |
2:55.36 |
-10.30 |
| 6 |
Peretti, Cameron S (11)
|
AMWHAT |
2:58.44 |
2:55.40 |
-3.04 |
| 7 |
Gregorowicz, Caroline O (11)
|
AMPRA |
3:10.67 |
2:57.07 |
-13.60 |
| 8 |
Klaum, Kaitlyn R (11)
|
AMSTEE |
2:56.03 |
2:57.53 |
1.50 |
| 9 |
Leehr, Abigail S (11)
|
AMPTSC |
3:04.21 |
2:57.99 |
-6.22 |
| 10 |
Josephson, Mara A (12)
|
AMSVSC |
2:53.44 |
2:59.55 |
6.11 |
| 11 |
Stoecklein, Caroline M (12)
|
AMSTEE |
3:01.27 |
3:00.10 |
-1.17 |
| 12 |
Kabala, Ryleigh D (11)
|
AMPRA |
3:10.67 |
3:06.53 |
-4.14 |
| 13 |
Daniele, Anabella N (11)
|
AMSVSC |
3:10.33 |
3:06.79 |
-3.54 |
| 14 |
Maxwell, Abby F (11)
|
AMSVSC |
3:15.01 |
3:11.53 |
-3.48 |
| 15 |
Liebman, Sophia B (12)
|
AMSVSC |
3:12.54 |
3:12.28 |
-0.26 |
| 16 |
Bowers, Taylor J (11)
|
AMPTSC |
3:17.61 |
3:13.24 |
-4.37 |
| 17 |
Danish, Julia N (12)
|
AMPTSC |
3:10.67 |
3:15.16 |
4.49 |
| 18 |
Visconti, Jordan L (11)
|
AM3RVS |
3:40.00 |
3:41.17 |
1.17 |
| 19 |
Santhanam, Dhara M (11)
|
AMPEAQ |
3:50.21 |
4:16.28 |
26.07 |