| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lowry, Melissa S (15)
|
MAUNGP |
24.58 |
24.80 |
0.22 |
| 2 |
Parker, Hailey A (16)
|
MAGPAC |
24.35 |
25.77 |
1.42 |
| 3 |
Jones, Madison A (16)
|
MAGPAC |
29.26 |
28.70 |
-0.56 |
| 4 |
Carapella, Rachel A (13)
|
MAGPAC |
28.28 |
28.76 |
0.48 |
| 5 |
Guilfoyle, Alexandra C (13)
|
MAGPAC |
30.07 |
29.47 |
-0.60 |
| 6 |
Martinez Delgadillo, Ilse A (17)
|
MAGPAC |
28.77 |
29.76 |
0.99 |
| 7 |
Klumpp, Ava C (14)
|
MAGPAC |
31.19 |
30.71 |
-0.48 |
| 8 |
Mesco, Caroline T (13)
|
MAGPAC |
|
30.84 |
- |
| 9 |
VanderNeut, Reilly A (13)
|
MAGPAC |
31.23 |
31.53 |
0.30 |
|
Fondacaro, Sarah M (14)
|
MAGPAC |
28.15 |
NS |
- |