| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cortes, Erin B (10)
|
MAJW |
3:02.29 |
3:05.10 |
2.81 |
| 2 |
Ryan, Maxalyn E (10)
|
MAWAC |
3:22.74 |
3:10.48 |
-12.26 |
| 3 |
Walsh, Ellie F (10)
|
MAECCC |
2:52.23 |
3:11.39 |
19.16 |
| 4 |
Keim, Lilliana E (10)
|
MASEA |
3:17.37 |
3:15.26 |
-2.11 |
| 5 |
Ashman, Aubrey V (9)
|
MAWAC |
3:18.55 |
3:16.77 |
-1.78 |
| 6 |
McGlynn, Shea (10)
|
MABLUE |
3:27.22 |
3:17.49 |
-9.73 |
| 7 |
Karasinski, Gabriella (9)
|
MAUN-1 |
3:23.35 |
3:17.88 |
-5.47 |
| 8 |
Kiess, Andie N (8)
|
MAUN-2 |
3:09.02 |
3:19.13 |
10.11 |
| 9 |
Derivaux, Audrey R (8)
|
MAJW |
2:59.06 |
3:20.90 |
21.84 |
| 10 |
Jung, Seo Y (10)
|
MACBST |
3:36.59 |
3:27.53 |
-9.06 |
| 11 |
Lehman, Alyce E (10)
|
MAJW |
3:04.71 |
3:27.71 |
23.00 |
| 12 |
Devlin, Mia L (9)
|
MAWAC |
3:11.77 |
3:29.33 |
17.56 |
| 13 |
Nalli, Alexia C (10)
|
MABLUE |
3:28.90 |
3:30.02 |
1.12 |
| 14 |
Hoffman, Alaina G (9)
|
MAWAC |
3:18.74 |
3:34.33 |
15.59 |
| 15 |
Moore, Violet E (10)
|
MAFCA |
3:40.67 |
3:35.26 |
-5.41 |
| 16 |
Kohm, Gabriella E (9)
|
MAGAAC |
3:24.03 |
3:51.30 |
27.27 |
| 17 |
Caraballo, Natalia R (10)
|
MAWAC |
3:28.42 |
4:01.74 |
33.32 |
|
Coombe, Taylor B (10)
|
MAPSC |
3:15.29 |
DQ |
- |