| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eliason, Sarah K (13)
|
PVRMSC |
24.37 |
24.80 |
0.43 |
| 2 |
Qin, Amy W (13)
|
PVRMSC |
24.40 |
24.86 |
0.46 |
| 3 |
Iwasyk, Isabel B (13)
|
MADST |
24.38 |
24.87 |
0.49 |
| 4 |
Redman, Emma L (13)
|
PVFISH |
25.21 |
25.73 |
0.52 |
| 5 |
Varis, Annika L (13)
|
PVRMSC |
26.15 |
26.31 |
0.16 |
| 6 |
Yo, Abigail S (13)
|
PVRMSC |
26.14 |
26.38 |
0.24 |
| 7 |
Fanelli, Lila R (13)
|
MADST |
26.77 |
26.72 |
-0.05 |
| 8 |
Phillips, Victoria L (13)
|
MADST |
27.23 |
27.03 |
-0.20 |
| 9 |
Carfi, Victoria (13)
|
MADST |
27.69 |
28.26 |
0.57 |
| 10 |
Boughanem, Sabrina L (13)
|
PVFISH |
28.54 |
28.39 |
-0.15 |
| 11 |
Sharma, Maya S (13)
|
PVFISH |
29.25 |
29.33 |
0.08 |