| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wong, Andrew L (18)
|
PVRMSC |
48.23 |
48.11 |
-0.12 |
| 2 |
Naulgan, Jarod Andwele C (18)
|
PVRMSC |
48.14 |
48.53 |
0.39 |
| 3 |
Webster, Jordan J (19)
|
PVRMSC |
48.31 |
49.04 |
0.73 |
| 4 |
Ginsburg, Jonah R (15)
|
PVRMSC |
48.69 |
49.22 |
0.53 |
| 5 |
Kalinin, Nikolai L (16)
|
PVRMSC |
49.43 |
50.08 |
0.65 |
| 6 |
Dolezel, Dominik M (15)
|
PVRMSC |
49.27 |
50.23 |
0.96 |
| 7 |
Devinney, Edward (15)
|
PVRMSC |
48.32 |
50.60 |
2.28 |
| 8 |
Conte, Antonio M (15)
|
PVRMSC |
52.16 |
51.07 |
-1.09 |
| 9 |
Franken, Evander (16)
|
PVRMSC |
51.26 |
51.29 |
0.03 |
| 10 |
Qian, Ethan (17)
|
PVRMSC |
51.87 |
51.30 |
-0.57 |
| 11 |
De Angelis, Pietro (15)
|
PVRMSC |
52.24 |
51.66 |
-0.58 |
| 12 |
Leeroy, Wesley (17)
|
PVRMSC |
51.67 |
51.97 |
0.30 |
| 13 |
Wang, William C (15)
|
PVRMSC |
51.84 |
52.19 |
0.35 |
| 14 |
Trachtenberg, Benjamin J (15)
|
PVRMSC |
52.38 |
53.19 |
0.81 |
| 15 |
Hua, Ethan (15)
|
PVRMSC |
51.94 |
53.38 |
1.44 |
| 16 |
Rook, William F (16)
|
PVRMSC |
52.30 |
53.65 |
1.35 |
| 17 |
Jones, Jacob M (17)
|
PVRMSC |
52.55 |
54.57 |
2.02 |
|
Conley, Padraig T (15)
|
PVRMSC |
52.47 |
DQ |
- |