| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Donoghue, Rowan B (12)
|
MADST |
25.09 |
25.16 |
0.07 |
| 2 |
Dworak, Andrea M (12)
|
PVRMSC |
25.93 |
25.79 |
-0.14 |
| 3 |
Schwieters, Julie K (12)
|
PVRMSC |
25.48 |
25.82 |
0.34 |
| 4 |
Wu, Jingjing (12)
|
PVRMSC |
26.36 |
26.20 |
-0.16 |
| 5 |
Depman, Megan A (12)
|
PVRMSC |
27.03 |
26.68 |
-0.35 |
| 6 |
Phillips, Alexandra J (12)
|
MADST |
28.57 |
27.33 |
-1.24 |
| 7 |
Watson, Marina M (12)
|
PVFISH |
27.09 |
27.62 |
0.53 |
| 8 |
O'Shaughnessy, Mary M (12)
|
PVFISH |
27.59 |
27.65 |
0.06 |
| 9 |
Zhou, Michelle (12)
|
MADST |
28.68 |
28.30 |
-0.38 |
| 10 |
Alms, Katherine J (12)
|
PVFISH |
28.56 |
28.77 |
0.21 |
| 11 |
Coughlin, Katherine E (12)
|
MADST |
29.53 |
29.25 |
-0.28 |