| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Isaac T (12)
|
NCCAT |
31.64 |
30.61 |
-1.03 |
| 1 |
Roberts, Christopher S (12)
|
NCCAT |
30.21 |
30.61 |
0.40 |
| 3 |
Villalobos, Adrian J (11)
|
NCRSA |
32.02 |
31.25 |
-0.77 |
| 4 |
Gray, William R (12)
|
NCCAT |
33.99 |
31.83 |
-2.16 |
| 5 |
Pritchard, Benjamin A (12)
|
NCCAT |
30.01 |
32.09 |
2.08 |
| 6 |
Johnson, Cameron M (12)
|
NCECA |
32.09 |
32.37 |
0.28 |
| 7 |
Czar, Matthew E (11)
|
NCHAC |
35.29 |
33.45 |
-1.84 |
| 8 |
Bullard, Collin M (12)
|
NCECA |
36.30 |
33.83 |
-2.47 |
| 9 |
Malone, Samuel M (12)
|
NCRSA |
33.51 |
34.59 |
1.08 |
| 10 |
Haithcock, William J (12)
|
NCRSA |
34.63 |
35.87 |
1.24 |
| 11 |
Frank, Jack P (12)
|
NCCAT |
34.10 |
36.08 |
1.98 |
| 12 |
Cole, Tyrus D (12)
|
NCECA |
36.11 |
37.55 |
1.44 |
| 13 |
Andre, Alex U (11)
|
NCHAC |
37.52 |
39.85 |
2.33 |
| 14 |
Jenkins, Ziyad S (11)
|
NCCAT |
42.07 |
40.06 |
-2.01 |
| 15 |
Marlow, Tiago V (12)
|
NCECA |
42.49 |
40.21 |
-2.28 |
| 16 |
Winslow, William C (12)
|
NCRSA |
42.74 |
43.08 |
0.34 |
| 17 |
Cook, Tyson G (12)
|
NCECA |
57.44 |
43.41 |
-14.03 |
| 18 |
Johnson, Samuel T (11)
|
NCECA |
41.58 |
43.48 |
1.90 |