| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bauerlein, John G (7)
|
PVNCAP |
44.01 |
41.60 |
-2.41 |
| 2 |
Diem, Arius-Saersng C (7)
|
PVNCAP |
|
42.05 |
- |
| 3 |
Mikhailov-Bakker, Andrei J (7)
|
PVNCAP |
|
43.08 |
- |
| 4 |
Klotz, Riley P (7)
|
PVBWST |
45.00 |
45.93 |
0.93 |
| 5 |
Kotowicz, Talan (7)
|
PVNCAP |
49.09 |
46.10 |
-2.99 |
| 6 |
Brado, Jonathan M (6)
|
PVNCAP |
|
46.73 |
- |
| 7 |
Conjura, Chase E (7)
|
PVNCAP |
46.45 |
47.07 |
0.62 |
| 8 |
Delaney, Jamison M (6)
|
PVBWST |
50.82 |
49.67 |
-1.15 |
| 9 |
Karp, Joel F (7)
|
PVNCAP |
|
51.68 |
- |
| 10 |
Johnson, Connor S (7)
|
PVNCAP |
|
53.04 |
- |
| 11 |
Sibole, Jasper S (6)
|
PVNCAP |
|
54.49 |
- |
| 12 |
Garbis, Brady J (7)
|
PVNCAP |
|
56.94 |
- |
|
Lincoln, David G (7)
|
PVSNOW |
|
NS |
- |