| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Anderson, Stella V (10)
|
NTNTN |
41.15 |
40.31 |
-0.84 |
| 2 |
Dukes, Kameron C (10)
|
NTNTN |
50.90 |
45.51 |
-5.39 |
| 3 |
Rockafellow, Charlie E (9)
|
NTNTN |
48.80 |
45.82 |
-2.98 |
| 4 |
Walser, Georgia H (10)
|
NTNTN |
53.86 |
49.06 |
-4.80 |
| 5 |
Franco, Aliana M (8)
|
NTNTN |
51.83 |
50.24 |
-1.59 |
| 6 |
DeYoung, Kay I (10)
|
NTNTN |
52.06 |
51.24 |
-0.82 |
| 7 |
Nguyen, Emma H (9)
|
NTNTN |
56.71 |
52.75 |
-3.96 |
| 8 |
McNary, Kameron K (10)
|
NTNTN |
|
52.99 |
- |
| 9 |
Smetana, Shelby T (8)
|
NTNTN |
58.07 |
57.43 |
-0.64 |
| 10 |
Weilert, Chandler R (9)
|
NTNTN |
56.66 |
59.34 |
2.68 |
| 11 |
Frost, Ava M (9)
|
NTNTN |
|
59.99 |
- |
| 12 |
Klopfenstein, Katelyn J (8)
|
NTNTN |
|
1:08.53 |
- |
|
Peel, Scarlett E (7)
|
NTNTN |
|
DQ |
- |
|
Palmer, Elizabeth J (8)
|
NTNTN |
50.65 |
NS |
- |